【摘要】 采用大孔吸附树脂-制备液相色谱联用技术分离制备杜仲醇。以杜仲叶为原料,采用超声波辅助提取,提取液经过正丁醇萃取、D101大孔吸附树脂柱分离纯化后,再通过反相半制备液相色谱分离,以V(甲醇):V(水)=15∶85为流动相进行洗脱,制备得到杜仲醇单体。采用紫外光谱(UV)、红外光谱(IR)、核磁共振波谱(NMR)及液相色谱-质谱联用(LC-MS)等方法对所得单体进行了结构验证。液相色谱法分析检测表明制备所得杜仲醇纯度达95.12%。更多还原

【Abstract】 In this study,eucommiol was isolated and purified from leaves of Eucommia ulmoides Oliv. by liquid-liquid extraction,macroporous resin adsorption and semi-preparative HPLC. Firstly,the crude extract was extracted by ultrasonicassisted extraction using n-butanol as extraction solvent. Secondly,the n-butanol extract was separated by D101 macroporous resin column. Finally,eucommiol was prepared by reverse phase semi-preparative HPLC. The mobile phase of semi-preparative HPLC was methanol-water( V∶ V = 15 ∶ 85). The structure of the isolated eucommiol was subsequently confirmed by ultraviolet spectrophotometry( UV),infrared absorption spectroscopy( IR),nuclear magnetic resonance spectroscopy( NMR) and liquid chromatography-mass spectrometry( LC-MS). HPLC analyses were performed to test and verify the purity of eucommiol( 95. 12%).更多还原