【摘要】 目的探索鱼精蛋白(PS)包覆聚乳酸(PLA)纳米囊对小鼠骨髓系树突状细胞(BMDC)体外免疫应答效应的影响。方法采用复乳溶剂挥发法制备包裹模型抗原鸡卵清白蛋白(OVA)的PLA纳米囊,以PS进行表面修饰。将纳米囊作用于体外培养的BMDC,用流式细胞仪检测BMDC表面分子CD80、CD83、CD86、MHCⅠ、MHCⅡ的表达和对纳米囊的摄取情况,用ELISA法检测BMDC分泌IL-12p70水平。结果 PS包覆PLA纳米囊可显著促进BMDC表达CD80、CD83、MHCⅠ和MHCⅡ表面分子,增加BMDC分泌IL-12p70,增强BMDC对OVA的摄取。结论PS包覆PLA纳米囊可以增强BMDC的免疫应答效果,有望成为一种良好的药物载体。更多还原

【Abstract】 Objective To investigate the effect of protamine(PS)coated polylactic acid(PLA)nanocapsule on the immune response of murine bone marrow-derived dendritic cells(BMDCs).Methods PLA nanocapsules were prepared by W1/O/W2 method.Protamine was used to modify the surface of PLA nanocapsule that encapsulated ovalbumin(OVA),a model antigen.The prepared OVA/PLA/PS nanocapsules were used to stimulate BMDCs.Flow cytometry was used to analyze the expression of surface molecules CD80,CD83,CD86,MHCⅠ,and MHC Ⅱ,and the uptake of nanocapsule.The levels of IL-12p70 secreted by BMDCs were detected by ELISA.Results OVA/PLA/PS nanocapsules could significantly up-regulate the expression of CD80,CD83,MHCⅠ,and MHC Ⅱin BMDCs,and increase the secretion of IL-12p70 by BMDCs.Furthermore,OVA/PLA/PS nanocapsules could enhance the uptake efficiency of OVA by BMDCs.Conclusion OVA/PLA/PS nanocapsule can enhance the immune response of BMDCs,and may become a good drug delivery carrier.更多还原