【摘要】 目的:研究ST7L基因对人类卵巢癌细胞系OVCAR3和SKOV3细胞增殖、细胞周期和体内裸鼠成瘤模型的影响。方法:PCR扩增ST7L过表达质粒(pc DNA3/ST7L),生物合成ST7L的敲降质粒(p Silencer/ST7L);利用脂质体转染技术在卵巢癌细胞中过表达或者敲降ST7L基因,用荧光定量PCR、Western blot验证质粒的有效性;MTT、克隆形成实验、流式细胞术检测ST7L对OVCAR3和SKOV3细胞增殖能力、周期的影响;并通过裸鼠移植瘤实验研究ST7L在体内成瘤能力。结果:过表达和敲降ST7L质粒是有效的;过表达ST7L后抑制了卵巢癌细胞生长,抑制了细胞G1/S期的转化;过表达ST7L在体内能够有效抑制裸鼠的成瘤能力。结论:ST7L抑制OVCAR3和SKOV3细胞的增殖能力,抑制细胞周期进程,抑制体内裸鼠成瘤能力。更多还原

【Abstract】 O bjective: To investigate the effects of ST7 L on the proliferation, cell cycle in ovarian cancer and tumor xenograft in nude mice. Methods: Ectopic expression plasmid of ST7 L by PCR and compounded the knockdown plasmid were established. ST7 L was overexpressed and knocked down in OVCAR3 and SKOV3 cells via lipidosome 2000 transfection. Fluorescence based quantitative polymerase chain reaction and western blot assay were used to analyze the expression of ST7 L. MTT, colony formation assay and flow cytometry were applied to detect the effects of ST7 L on the proliferation and cell cycle of OVCAR3 and SKOV3 cells after transfection. Tumor xenograft studies were used to observe the tumor growth in vivo. Results: The used plasmids were effective; the ectopic expression of ST7 L suppressed the proliferation and cell cycle of OVCAR3 and SKOV3 cells, and the tumor growth was inhibited by over-expression of ST7 L in vivo.Conclusion:ST7L could inhibit the proliferation and cell cycle of ovarian cells and tumor growth in nude mice.更多还原