【摘要】 利用气相色谱-质谱联用(GC-MS)探讨解淀粉芽孢杆菌(Bacillus amyloliquefaciens)LJ02菌株在LB固体培养中产生挥发性气体的种类,并以5种病原真菌为指示菌,用平板对扣法和分格平板法检测LJ02菌株产生的挥发性气体的抑菌活性,确定对黑腐皮壳菌(Valsa mali)抑制作用的有效气体成分。结果表明:解淀粉芽孢杆菌LJ02产生的挥发性气体对多种果类致病菌,特别是对黑腐皮壳菌(V.mali)、草莓灰霉病菌(Botrytis cinerea)、枣浆胞病菌(Alternaria spp.)有显著抑制效果,抑菌率在50%以上。LJ02菌株在代谢过程中可产生多种挥发性气体,其中4-乙基苯酚、2-丙基环己酮2种气体对黑腐皮壳菌(V.mali)有强烈的抑制效果。更多还原

【Abstract】 The paper used GC-MS to identify the kinds of volatile products in LB solid-state cultivation of B. amyloliquefaciens LJ02, and took 5 kinds of pathogenic fungi as indicator bacteria with two-sealed-base-plates method and two compartment plate method to detect the antibacterial activity of volatile gas produced by LJ02 strain and determined which active gas ingredients had inhibition with V. mali. The results showed that the volatile products in LB solid state cultivation of B. amyloliquefaciens LJ02 had significant inhibitory effect on most of the fruit-causing fungus, especially V. mali, B. cinerea and Alternaria spp., the inhibitory rate reaching above 50%. B. amyloliquefaciens LJ02 in metabolic process can produce a variety of volatile gases, including 4-ethylphenol, 2-propylcyclohexanone have strong inhibitory effect on V. mali.更多还原