Young wheat spikes of 2～3 cm in length were cut into 2～3 mm segments,which were then inoculated on the MS induction medium plus 2 mg·L -1 2,4 D,0.5 mg·L -1 NAA, 30 g·L -1 sucrose.When the induced Calli grew into about 5 mm in diameter,they were transferred directly onto differentiation mediam,from which 54 abnormal spikelets were obtained.These spikelets were fasciculate or branchlike,had acomplete floral organs which are 2～8 times larger than normal spikelets insize,and their glumes and rac...
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