【摘要】 建立了同时测定饮料中5种食用合成色素的在线固相萃取-高效液相色谱-紫外检测(Online-SPE-HPLC-UV)方法。饮料样品用水稀释并经0.45μm微孔滤膜过滤后,在线固相萃取柱(Acclaim 120 C18)净化,反相C18分析柱分离,梯度洗脱,紫外检测器检测。结果表明:5种食用合成色素在0.5~20 mg/L浓度范围内线性关系良好,相关系数(r2)均大于0.999 9,检出限为0.02~0.08 mg/kg,3个水平下的加标回收率为94.5%~103.0%,相对标准偏差为0.4%~2.7%。该研究为快速准确地分离测定饮料中的食用合成色素提供了有效方法。更多还原

【Abstract】 A method was developed for the determination of 5 kinds of synthetic pigments in drinks by online solid-phase extraction coupled with high performance liquid chromatography-ultraviolet detection( online-SPE-HPLC-UV). The drinks samples were diluted with water,and filtered with0. 45 μm microporous membrane filter. Then the samples were purified on an Acclaim 120 C18 column and separated on a reversed-phase column by gradient elution. The detection was performed by ultraviolet detection. The calibration curves of 5 kinds of synthetic pigments were linear in the concentration range of 0. 5-20 mg/L,with correlation coefficients( r2) greater than 0. 999 9. The limits of detection( LODs) were in the range of 0. 02-0. 08 mg/kg. The recoveries of 5 kinds of synthetic pigments at three spiked levels ranged from 94. 5% to 103. 0%,with relative standard deviations of 0. 4%-2. 7%. The research provides an effective method for the rapide determination of edible synthetic pigments in drinks.更多还原