【摘要】 运用一种新型的化学引发-电聚合方式将中性红膜固定到碳糊电极表面,制备出聚中性红薄膜修饰碳糊电极（PNR/CPE）。利用循环伏安法（CV）和交流阻抗谱（EIS）对修饰电极的电化学性能进行研究,借助于扫描电子显微镜（SEM）对修饰电极表面进行表征,并采用红外吸收光谱法（IR）和紫外可见吸收光谱法（UV-Vis）对PNR薄膜结构进行测试。结果表明,中性红成功地固定在碳糊电极表面,修饰电极的表面呈现特定的立体化结构,表面的电活性位点增多,电催化性能增大。在优化条件下,将该电极应用于鲱鱼精DNA（hs DNA）的检测,PNR电极上出现了1对较强的氧化还原峰,峰电流与其浓度在1.0×10^-68.0×10^-5mol/L范围内呈良好的线性关系,检出限为1.0×10^-7mol/L。更多还原

【Abstract】 A novel poly neutral red film modified carbon paste electrode（ PNR / CPE） was prepared by chemical initiation- electrochemical polymerization. The electrochemical properties of the modified electrode were characterized by cyclic voltammetry（ CV） and electrochemical impedance spectroscopy（ EIS）,the surface morphology of the ploy neutral red film was observed by scanning electron microscopy（ SEM）. Meanwhile,the membrane structure of PNR was tested and analyzed by ultraviolet visible absorption（ UV- Vis） and infrared absorption spectrometry（ IR）. The result showed that the neutral red was modified successfully on the surface of carbon paste electrode,and the surface of PNR / CPE had a specific three-dimensional structure,in which the electroccatalytic performance was improved with the increase of the electroactive sites of the surface. Under the optimal experimental conditions,the modified electrode was applied in the detection of herring sperm DNA（ hs DNA）. The result indicated that a pair of obvious redox peak appeared on the PNR / CPE,the peak currents and concentrations of hs DNA had a good linear relationship in the range of 1. 0 × 10^{- 6}- 8. 0 × 10^{- 5}mol / L with a detection limit of 1. 0 × 10^{- 7}mol / L.更多还原