【摘要】 目的观察制首乌总游离蒽醌在防治兔颈动脉粥样硬化过程中对Caspase-3,8,9 mRNA表达的影响。方法将36只雄性新西兰大白兔随机分成6组:正常对照组、模型组、血脂康组和制首乌总游离蒽醌高、中、低剂量组。免疫损伤法结合高脂饲料喂养建立AS模型,造模周期为12周。用制首乌总游离蒽醌i.g给药4周后,耳缘静脉取血离心,检测血脂水平;麻醉动物后,取颈动脉,用RT-PCR法检测颈动脉中Caspase-3,8,9 mRNA的表达。结果与模型组比,制首乌总游离蒽醌能降低TG的含量,高剂量组P<0.05,中、低剂量组P<0.01,TC、LDL的含量高、中、低剂量组均有显著降低P<0.01,均具有统计学意义;与模型组比,制首乌总游离蒽醌能降低Caspase-3,8,9 mRNA的表达水平,高剂量组P<0.01,而中、低剂量组P<0.05,均具有统计学意义;病理切片结果显示,制首乌总游离蒽醌能延缓动脉粥样硬化斑块的形成。结论制首乌总游离蒽醌具有抑制细胞凋亡延缓动脉粥样硬化作用,该作用机制可能与其可能下调Caspase-3,8,9mRNA的表达有关。更多还原

【Abstract】 Objective To observe the influence of free anthraquinone in Fallopia Multiflora in prevention and treatment of rabbit carotid artery atherosclerotic process for expression of Caspase-3,8 or 9 mRNA. Methods 36 male New Zealand white rabbits were randomly divided into 6 groups: normal group,model group,Hypo Col group,Radix Polygoni Multiflori total free anthraquinone high,medium,low dose group. Immune damage method combined with high fat feed establish atherosclerosis model,building cycle for 12 weeks. Then with total free anthraquinone of Radix Polygoni Multiflori by intragastric administration for animal.To extract the ear marginal vein and centrifugation after 4 weeks,then detect the serum cholesterol levels. After anesthesia animals,shear the cervical aortic,detect the expression of Caspase-3,8 or 9 mRNA in cervical aortic by RT-PCR method. Results Compared with model group,Radix Polygoni Multiflori total free anthraquinone can reduce the levels of TG,the high dose group( P < 0. 05),middle and low dose group( P < 0. 01),the TC and LDL levels of high,medium and low dose group were significantly lower( P < 0. 01),haven statistically significant; With the model group than that of Radix Polygoni Multiflori total free anthraquinone can reduce Caspase-3,8 or 9 mRNA expression level,high dose group( P < 0. 01),while middle and low dose group( P < 0. 05),statistically significant; Pathological section results show that the total free anthraquinone of Radix Polygoni Multiflori can slow down the formation of atherosclerotic plaque. Conclusion The Radix Polygoni Multiflori total free anthraquinone can restrain apoptosis and slow down atherosclerosis,It’s possible to downgrade Caspase-3,8 or 9 mRNA expression of the mechanism of action.更多还原