【摘要】 目的检测大肠癌组织中CD133、COX-2的表达和DNA倍体状态,探讨这些因素与大肠癌临床病理特征的关系及彼此间的相关性。方法采用免疫组织化学SP法对90例大肠癌组织和90例大肠正常黏膜组织进行CD133、COX-2检测,同时运用流式细胞术对其中30例进行DNA倍体检测。结果CD133、COX-2的表达及DNA异倍体在大肠癌中显著高于大肠正常黏膜组织(P<0.05);其中CD133在大肠癌中的表达与组织分化程度及淋巴结转移呈正相关(P<0.05),COX-2在大肠癌中的表达与TNM分期及淋巴结转移呈正相关(P<0.05)。此外,CD133、COX-2及DNA倍体在大肠癌中彼此间也呈正相关(P<0.01)。结论大肠癌中CD133、COX-2的表达及DNA倍体增加,且彼此间呈正相关性;暗示着这些指标可能与大肠癌的发生、发展密切相关,可能作为临床治疗和研究的重要依据。更多还原

【Abstract】 Objective To detect the expression of CD133,COX- 2 and the state of DNA ploidy in colorectal carcinoma;to investigate the relationship of between these factors and clinicopathological characteristics of colorectal carcinoma,and the correlationship between of these factors.Methods Immunohistochemical SP method was performed to detect CD133 and COX-2 in 90 cases of colorectal carcinoma and in 90 cases of normal intestinal mucosal,the detection of DNA ploidy was examined by flow cytometry method(FCM).Results The positive expression of CD133,COX-2 and DNA ploidy in colorectal cancer were significant higher than that in normal intestinal mucosa(P <0.05);the expression of CD133 was positive correlated to the degree of differentiation and lymphoid metastasis in colorectal cancer(P <0.05);and the expressions of COX-2 was positive correlated to TNM staging and lymphoid metastasis(P <0.05);the positive expression of CD133,COX-2 and DNA ploidy rates were positive correlated with each other(P <0.01).Conclusion The CD133,C0X-2 and DNA ploidy in colorectal carcinoma were high expressed and positive correlated with each other,these results indicated that these factors may relate with the occurrence and development of colorectal cancer,and may provide an important basis for the clinical treatment and research.更多还原