【摘要】 目的:检测增殖诱导配体(APRIL)在尿路上皮癌组织中的表达,研究它在尿路上皮癌组织和癌旁组织中的表达差异,分析APRIL与尿路上皮癌临床病理特征的关系。方法:收集住院的尿路上皮癌患者术后癌组织33例及癌旁组织23例,采用实时荧光定量PCR(q RT-PCR),免疫组织化学染色及Western blot方法检测APRIL的表达水平,分析APRIL的表达与尿路上皮癌临床病理的关系。结果:q RT-PCR结果显示,APRIL m RNA在尿路上皮癌组织中表达量显著高于癌旁组织中的表达(P<0.05);免疫组化结果显示,APRIL在尿路上皮癌组织中上调表达,在癌旁组织中表达较低;Western blot检测发现尿路上皮癌组织中APRIL蛋白表达水平明显高于癌旁组织(P<0.05);统计学分析发现APRIL在尿路上皮癌组织中的高表达与患者的性别、年龄、肿瘤大小、淋巴结是否转移及临床分期无相关性(P>0.05)。结论:APRIL在尿路上皮癌组织中异常高表达,可能与尿路上皮癌的发生发展相关。更多还原

【Abstract】 Objective To investigate the expression of a proliferation-inducing ligand(APRIL) in urothelial carcinoma tissue and its clinical significance.Methods We investigated the expression of APRIL mRNA in urothelial carcinoma tissue of 33 patients in urothelial carcinoma in Cangzhou Central Hospital from July2013 to March 2015,Among of them,23 cases of adjacent pericancerous tissues were detected by qRT-PCR.Immunohistochemistry and western blot were used to detect the APRIL protein expression level in urothelial carcinoma tissue and adjacent pericancerous tissues.We analyzed the relationship between APRIL expression and clinical pathology in patients with urothelial carcinoma by statistical methods.Results The qRT-PCR revealed that the expression of APRIL in urothelial carcinoma tissue was significantly higher than that in the adjacent pericancerous tissues(P < 0.05).Immunohistochemistry and western blot showed that the expression of APRIL in urothelial carcinoma tissue was higher than that in the adjacent pericancerous tissues.Correlation analysis found that the high expression of APRIL in urothelial carcinoma tissue was not significantly related to gender,age,size of tumor,lymph node metastasizing or not,and clinical stages(P > 0.05).Conclusion The APRIL is a high abnormal expression in urothelial carcinoma tissues,and maybe related to the occurrence and development of urothelial carcinoma.更多还原