【摘要】 目的:研究三氧化二砷(ATO)联合Rad001对卵巢癌细胞株SKOV3细胞凋亡和自噬的影响,探讨可能的分子机制。方法:ATO和Rad001分别单独或联合作用于SKOV3细胞后,细胞荧光定量活性检测评估联合用药效应指数。流式细胞仪检测凋亡率,Western blot检测自噬蛋白和凋亡相关蛋白表达。结果:与单独用药组相比,联合组(ATO和Rad001)可协同显著降低卵巢癌活性细胞数、诱发其过度自噬和引起卵巢癌细胞凋亡增加(P<0.05)。结论:ATO联合Rad001可协同诱导的卵巢癌SKOV3细胞凋亡,其效应可能通过引起卵巢癌SKOV3细胞过度自噬实现。更多还原

【Abstract】 Objective To investigate the synergistic anticancer effect of ATO combined with RadOOl on human ovarian cancer cells in vitro and the underlying mechanisms.Methods SKOV3 cells were treated with Rad001,ATO and a combination of Rad001 and ATO,respectively.Cell relative luminescence units,viability,and combination index were tested.The apoptosis was quantified by Flow cytometry.Autophagy and apoptosis associated proteins were measured with immunoblotting.Results Significant decrease in live cell number were observed in cells treated with combination of Rad001 and ATO,compared with single compound treatment(P <0.05).Moreover,a higher rates of autophagy as well as apoptosis were observed in the combination treatment compared with single compound treatment(P < 0.05).Conclusions combination of Rad001 and ATO can result in synergistic cytotoxicity through activation of the excessive autophagy and apoptotic pathway.更多还原