【摘要】 目的:研究CUEDC2对单侧输尿管梗阻(UUO)小鼠肾组织炎症反应及间质纤维化的作用。方法:Balb/c小鼠30只,随机分为假手术组、UUO组和CUEDC2治疗组。HE及Masson染色观察肾脏病理改变;ELISA法检测炎症因子表达;免疫组化法检测肾组织CUEDC2表达;Western Blot检测CUEDC2、纤连蛋白(Fibronectin)、钙黏蛋白(E-cadherin)、胶原蛋白I型(Collagen Ⅰ)表达。结果 :术后7、14 d,CUEDC2治疗组的肾间质纤维化相对面积、炎症因子ICAM1、MCP1、IL-1、IL-8表达及Fibronectin、CollagenⅠ表达均较UUO组明显减少(P<0.05),E-cadherin表达明显增加(P<0.05)。结论:CUEDC2可以抑制多种炎症因子表达,减少间质胶原沉积,减轻肾脏纤维化。更多还原

【Abstract】 Objective To study the effects of CUEDC2 on renal interstitial fibrosis and inflammation response in rats with unilateral ureteral obstruction(UUO). Methods 30 Balb / c rats were randomly distributed into sham operation group(sham-vector),uuo operation group(uuo-vector) and CUEDC2 treatment group after uuo(uuo-cuedc2). Hematoxylin-eosin and Masson staining were used to measure renal pathology; Inflammation factors were quantified by ELISA; Immunohistochemistry was performed to measure the expression of CUEDC2;Protein expression of CUEDC2, Fibronectin, E-cadherin, Collagen I were detected by Western Blot. Results At 7 and 14 d after operation, the area of interstitial fibrosis and expression of ICAM1,MCP1,IL1,IL8,Fibronectin and Collagen I in uuo-cuedc2 showed a marked decrease when compared to uuo-vector(p? 0.05),the level of E-cadherin was significantly increased(P < 0.05). Conclusion CUEDC2 can inhibit renal interstitial fibrosis and decrease the expression of inflammation factors and Collagen deposition.更多还原