【摘要】 目的:探讨叉头框转录因子C1(FOXC1)对鼻咽癌5-8F细胞株生物学行为的影响。方法:利用Western blot方法检测FOXC1在永生化人鼻咽部细胞株NP69、鼻咽癌低转移潜能细胞株6-10B、鼻咽癌高转移潜能细胞株5-8F中的表达;应用si RNA干扰FOXC1在鼻咽癌5-8F细胞株中的表达,观察其迁移和侵袭能力的变化。结果:Western blot结果显示FOXC1蛋白水平在细胞株NP69、细胞株6-10B及细胞株5-8F中的相对表达量分别为0.27±0.04、0.72±0.06及1.08±0.05,差异具有统计学意义(P<0.05);5-8F细胞株中FOXC1表达下调后划痕实验显示其迁移能力、Transwell小室显示其侵袭能力均明显减弱(P<0.05),且5-8F细胞株中Fibronectin和Vimentin蛋白的相对表达量均明显降低(P<0.05)。结论 :FOXC1可能通过改变Fibronectin、Vimentin的表达调节鼻咽癌的侵袭和转移能力,FOXC1可能成为分子治疗鼻咽癌的靶点。更多还原

【Abstract】 Objective To investigate the Influence of FOXC1 downregulating on biological behavior of nasopharyngeal carcinoma(NPC) 5-8F cell lines. Methods Western blotting was used to detect the protein levels of FOXC1 in human immortalized nasopharyngeal cell lines NP69, nasopharyngeal carcinoma cell lines 6-10 B with lower metastasis potential and nasopharyngeal carcinoma cell lines 5-8F with higher metastasis potential.si RNA was used to downregulate the expression of FOXC1 in 5-8F cell lines, and then ability of migration and invasion were observed. Results FOXC1 protein expression level in NP69 cell lines, 6-10 B cell lines and 5-8F cell lines were(0.27 ± 0.04),(0.7 ± 20.06),(1.08 ± 0.05) respectively, and the difference was statistically significant(P < 0.05). Also ability of cell migration and invasion significantly weakened after FOXC1 depletion in 5-8F cell lines(P < 0.05). Conclusion FOXC1 might regulate invasion and metastasis of nasopharyngeal carcinoma through changing expression of Fibronectin and Vimentin, and FOXC1 may be an available target for molecular target therapy of nasopharyngeal carcinoma.更多还原