【摘要】 目的:探讨砷暴露对不同发育阶段小鼠仔鼠海马CaMKⅡ表达水平的影响及可能的机制。方法:建立不同发育阶段小鼠仔鼠砷暴露模型（0、15、30和60 mg/L Na AsO₂组）,分别于仔鼠出生后（postnatal day,PND）第10、20和40天取脑组织并分离海马,采用real-time RT-PCR法检测CaMKⅡ亚单位CaMKⅡα和CaMKⅡβmRNA的表达水平。结果:PND10和PND20,不同剂量砷暴露组仔鼠海马CaMKⅡα和CaMKⅡβmRNA表达水平与对照组比较差异无统计学意义;PND40,不同剂量砷暴露组仔鼠海马CaMKⅡαmRNA表达水平均显著低于对照组,30和60 mg/L Na AsO₂组仔鼠海马CaMKⅡβmRNA表达水平均明显低于对照组（P<0.05）。结论:砷暴露可使小鼠仔鼠海马CaMKⅡ亚单位mRNA的表达水平降低,从而可能影响仔鼠的学习记忆能力。更多还原

【Abstract】 Objective:To investigate the impairment mechanism of learning and memory ability induced by arsenite throughstudying the effects of arsenite on the expression level of CaMK Ⅱ in the hippocampus of offspring mice at different developmentalstages. Methods:The model of offspring mice exposed to arsenite（0,15,30 and 60 mg/L Na AsO₂） was set up. In postnatal day（PND） 10,20 and 40,the expression levels of CaMKⅡα and CaMKⅡβ mRNA in the hippocampus were measured by real-timeRT-PCR. Results: In PND 10 and PND 20, the expression levels of CaMK Ⅱ α and CaMK Ⅱ β mRNA in the hippocampus ofoffspring mice exposed to arsenite had no significant difference compared with those in control group. In PND 40, the expressionlevels of CaMK Ⅱ α mRNA in the hippocampus of offspring mice exposed to arsenite were significantly lower than those in controlgroup, the expression levels of CaMK Ⅱ β mRNA in the hippocampus of offspring mice exposed to 30 and 60 mg/L arsenite weresignificantly lower than those in control group（P<0.05）. Conclusion: Arsenite can affect the expression levels of CaMK Ⅱsubunits in the hippocampus of offspring mice and further may impair the learning and memory ability.更多还原