【摘要】 目的探讨白细胞介素-23(IL-23)、IL-23受体(IL-23R)基因rs11209032位点单核苷酸多态性与强直性脊柱炎(AS)的关系。方法选取五邑地区汉族人群AS患者132例和同期健康体检者102名,采用酶联免疫吸附试验(ELISA)法检测血清中IL-23的表达水平,采用Taq Man探针对IL-23R基因rs11209032位点进行聚合酶链反应(PCR)分型。结果 AS患者外周血IL-23表达水平显著高于对照组(P<0.05);在五邑地区汉族人群中,AA,AG,GG基因型频率在AS中分别为5.3%,91.7%,3.0%,与对照组分布(20.6%,65.7%,13.7%)相比,差异有统计学意义(χ~2=24.63,P<0.05),AS患者和对照组中rs11209032位点A等位基因频率(51.1%,53.4%)和G等位基因频率(48.5%,46.6%)的分布差异无统计学意义(χ2=0.243,P>0.05);其各个基因型组的红细胞沉降率和C反应蛋白水平差异无统计学意义(P>0.05)。结论 IL-23R单核苷酸多态性rs11209032与本地人群AS发病有一定的相关性。更多还原

【Abstract】 Objective To investigate the association between interleukin(IL)-23,interleukin-23 receptor(IL-23R)gene rs11209032 single nucleotide polymorphism(SNP)and ankylosing spondylitis(AS)in Chinese Han patients.Methods One hundred and thirty two patients with AS and 102 healthy donors were studied, enzyme linked immunosorbent assay(ELISA) was used analyze the levels of IL-23,The genotypes of IL-23 R SNP was detected by Taq Man probe assays. Results The levels of the peripheral blood IL-23 were significantly higher in AS patients than those in normal controls(P<0.05);The rs11209032 genotypes in AS patients consisted of homozygote A/A(5.3%),A/G(91.7%)and G/G(3.0%),while The rs11209032 genotypes in the controls were composed of A/A(20.6%),A/G(65.7%)and G/G(13.7%). The significant difference was found in the distribution of rs11209032 genotypes between these two groups( χ2=24.63,P <0.05). But the frequency of rs11209032 allele in AS patients was not significantly changed when compared with the control group(χ~2=0.243,P>0.05). Conclusion There may be existence association between the IL-23R gene rs11209032 SNP and ankylosing spondylitis in local area population.更多还原