【摘要】 目的探讨α-干扰素(IFN-α)联合5-氟尿嘧啶(5-Fu)对人肝癌细胞Hep G2增殖及凋亡的影响及机制。方法培养人肝癌细胞Hep G2,实验分为4组,对照组不加药物干预,5-Fu组加入40μg/ml 5-Fu,IFN-α组加入4 000 U/ml IFN-α,联合用药组加入40μg/ml 5-Fu和4 000 U/ml IFN-α。培养48 h后收集细胞,采用MTT法检测各组细胞增殖,流式细胞仪检测各组细胞周期及凋亡情况,细胞免疫化学法检测各组Bcl-2、Caspase-3蛋白表达。结果 IFN-α组抑制作用较弱,为3.73%,5-FU组抑制率为22.50%,联合用药组抑制作用显著增强,抑制率为63.65%;联合用药组对肝癌Hep G2细胞的周期影响最大,G0/G1期比率明显增加,为(80.10±4.33)%,S期细胞比率明显减少,为(18.76±3.47)%,细胞增殖指数(PI)最低,为(20.11±4.53)%,差异有统计学意义(P<0.05);联合用药组肝癌Hep G2细胞凋亡率为(22.51±4.41)%,明显高于其他组(P<0.05);联合用药组Bcl-2蛋白表达率为(10.24±2.30)%,明显低于其他组(P<0.05);联合用药组Caspase-3蛋白表达率为(30.12±1.81)%,明显高于其他组(P<0.05)。结论 IFN-α联合5-FU能明显抑制Hep G2细胞增殖并诱导凋亡,其机制可能是通过调节Bcl-2、Caspase-3蛋白而发挥作用。更多还原

【Abstract】 Objective To investigate effect of the alpha- interferon( IFN- α) combined with 5- fluorouracil( 5- Fu) on proliferation and apoptosis of human hepatocellular carcinoma Hep G2 cells and its mechanism. Methods Cultured Hep G2 human hepatoma cells,were divided into4 groups. No drug intervention was added to the control group,40 μg / ml 5- Fu was added to the 5- Fu group,4 000 U / ml IFN- α was added to the IFN- α group,and 40 μg / ml 5- Fu,4 000 U / ml IFN- α was added to the combination group. After 48 h culture,the cells were collected,and cell proliferation was detected by MTT method,the cell cycle and apoptosis were detected by flow cytometry,the expression of Bcl- 2 and Caspase-3 in each group were detected by immunocytochemistry. Results IFN- group inhibitory effect was weak,3. 73%,and 5- FU group inhibition rate was 22. 5%. the combination group inhibition significantly enhanced,the inhibition rate was 63. 65%; The effect of combination group on the Hep G2 cell cycle was the most,the proportion of G0 / G1 phase increased significantly,was( 80. 10 ± 4. 33) %,the percentage of cells in S phase significantly reduced,was( 18. 76 ± 3. 47) %,cell proliferation index( PI) was lowest,was( 20. 11 ± 4. 53) %,the difference had statistical significance( P< 0. 05); The apoptosis rate of Hep G2 cells in combination group was( 22. 51 ± 4. 41) %,which was significantly higher than that in other groups( P< 0. 05); The expression rate of Bcl- 2 protein in the combination treatment group was( 10. 24 ± 2. 30) %,significantly lower than that in other groups( P < 0. 05). The expression rate of Caspase- 3 protein in the combination group was( 30. 12 ± 1. 81) %,was significantly higher than that in other groups( P < 0. 05). Conclusion IFN- α combined with 5- FU can obviously inhibit the proliferation and induce apoptosis of Hep G2 cells,the mechanism may be by regulating Bcl- 2,Caspase- 3 proteins play a role.更多还原