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Naa10p表达对舌鳞癌Tca8113细胞平阳霉素敏感性的影响

The effect of Naa10p on the sensitivity of tongue squamous cell carcinoma Tca8113 cells to pingyangmycin

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【作者】 郑军杨洋徐江赵娟张凯楠曾妍

【Author】 ZHENG Jun;YANG Yang;XU Jiang;ZHAO Juan;ZHANG Kainan;ZENG Yan;Departmentof Stomatology,The First Affiliated Hospital,School of Medicine,Shihezi University;Key Laboratory of Xinjiang Endemic and Ethnic Diseas,School of Medicine Shihezi University;

【机构】 石河子大学医学院第一附属医院口腔科石河子大学新疆地方与民族高发病教育部重点实验室·石河子大学医学院生化教研室

【摘要】 目的:观察N-α乙酰基转移酶(Naa10p)表达对舌鳞癌Tca8113细胞平阳霉素(PYM)敏感性的影响。方法:采用慢病毒体系分别构建干扰及过表达Naa10p的Tca8113细胞株,并设立相应的对照细胞Tca8113-LV-NC,鉴定干扰和过表达效率,MTS法检测药物处理后各处理组细胞对PYM的敏感性。结果:干扰Naa10p的Tca8113-LV-sh Naa10p细胞、过表达Naa10p的Tca8113-LV-Naa10p细胞与对照细胞Tca8113-LV-NC对平阳霉素的半数抑制浓度(IC50)分别为(20.772±0.106)μg/ml、(2.157±0.123)μg/ml、(6.301±0.069)μg/ml(组间比较,P<0.05)。结论:下调Naa10p表达可降低口腔鳞癌细胞Tca8113对平阳霉素的敏感性,上调Naa10p表达可增强口腔鳞癌细胞Tca8113对平阳霉素的敏感性。

【Abstract】 Objective: To study the effect of N-acetyltransferase 10( Naa10p) expression on the sensitivity of tongue squamous cell carcinoma Tca8113 cells to pingyangmycin( PYM). Methods: The lentiviral vectors of LV-sh Naa10 p,LV-Naa10 p and control LVNC were transfected into Tca8113 cells,the infection efficiency were identified,then the sensitivity of the infected cells to PYM was determined by MTS assay. Results: The IC50 of Tca8113-LV-sh Naa10 p group,Tca8113-LV-Naa10 p group and Tca8113-LV-NC group were( 20. 772 ± 0. 106) μg / ml,( 2. 157 ± 0. 123) μg / ml and( 6. 301 ± 0. 069) μg / ml respectively( between groups P < 0. 05). Conclusion: Knock down of Naa10 p may reduce and over expression of Naa10 p can increase the sensitivity of Tca8113 cells to PYM.

【基金】 国家自然科学基金(编号:81560473,81560442);兵团基金资助项目(编号:2014BB021,2015AD003);石河子大学高层次人才科研资金专项资助项目(编号:RCZX201330)
  • 【文献出处】 实用口腔医学杂志 ,Journal of Practical Stomatology , 编辑部邮箱 ,2016年03期
  • 【分类号】R739.86
  • 【被引频次】3
  • 【下载频次】105
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