【摘要】 目的:观察N-α乙酰基转移酶(Naa10p)表达对舌鳞癌Tca8113细胞平阳霉素(PYM)敏感性的影响。方法:采用慢病毒体系分别构建干扰及过表达Naa10p的Tca8113细胞株,并设立相应的对照细胞Tca8113-LV-NC,鉴定干扰和过表达效率,MTS法检测药物处理后各处理组细胞对PYM的敏感性。结果:干扰Naa10p的Tca8113-LV-sh Naa10p细胞、过表达Naa10p的Tca8113-LV-Naa10p细胞与对照细胞Tca8113-LV-NC对平阳霉素的半数抑制浓度(IC50)分别为(20.772±0.106)μg/ml、(2.157±0.123)μg/ml、(6.301±0.069)μg/ml(组间比较,P<0.05)。结论:下调Naa10p表达可降低口腔鳞癌细胞Tca8113对平阳霉素的敏感性,上调Naa10p表达可增强口腔鳞癌细胞Tca8113对平阳霉素的敏感性。更多还原

【Abstract】 Objective: To study the effect of N-acetyltransferase 10( Naa10p) expression on the sensitivity of tongue squamous cell carcinoma Tca8113 cells to pingyangmycin( PYM). Methods: The lentiviral vectors of LV-sh Naa10 p,LV-Naa10 p and control LVNC were transfected into Tca8113 cells,the infection efficiency were identified,then the sensitivity of the infected cells to PYM was determined by MTS assay. Results: The IC50 of Tca8113-LV-sh Naa10 p group,Tca8113-LV-Naa10 p group and Tca8113-LV-NC group were( 20. 772 ± 0. 106) μg / ml,( 2. 157 ± 0. 123) μg / ml and( 6. 301 ± 0. 069) μg / ml respectively( between groups P < 0. 05). Conclusion: Knock down of Naa10 p may reduce and over expression of Naa10 p can increase the sensitivity of Tca8113 cells to PYM.更多还原