【摘要】 目的 探讨T1ρ成像技术在淫羊藿治疗兔膝软骨早期退变中的监测作用，推测淫羊藿治疗骨关节炎（OA）的疗效机理。方法 建立早期OA模型：将28只实验兔，随机分为A、B、C 3组，A、B组各11只为处理组，右膝关节腔内注射0.5 mL 1.6%的木瓜蛋白酶溶液，建立早期关节炎，左膝注射等量稀释液作为对照，C组6只为空白组。初次注药3周后对A、B、C 3组行双膝MR矢状位T₂WI、3DFSSPGR、T1ρ序列扫描，并于A、B组内随机取2只行双膝病理检查，确定早期OA模型建立。干预模型：A组给予淫羊藿灌胃2月，B组给予等剂量无菌生理盐水灌胃2月，期间于0、1、2月分别对3组同时行MR检查。末次扫描后处死所有试验兔并取双膝股骨髁软骨做组织病理学检查。统计分析干预前后的关节软骨厚度、T1ρ值的组内及组间差异，并与组织病理学结果对照。结果 （1）关节腔内注射微量木瓜蛋白酶3周后可诱导早期兔膝骨关节炎模型建立，A、B组诱导侧T1ρ值均高于对照侧（P<0.05）,软骨厚度值无明显差异（P>0.05）;（2）淫羊藿干预组诱导侧软骨T1ρ值下降（P<0.05）,盐水干预组T1ρ值无明显变化（P>0.05）。结论 （1）OA早期、进展期软骨T1ρ值不同程度升高，升幅与软骨基质内成分比例相关；（2）病理证实单味中药淫羊藿治疗早期兔膝骨关节炎有效，推测其可能机制为干预早期OA发展过程中的共同通道（蛋白酶软骨基质降解）,抑制蛋白酶形成并促进软骨基质中蛋白多糖分泌。更多还原

【Abstract】 Objective To explore the role of MRI（T1ρ）in the early osteoarthritis,curative efficacy monitoring and speculated that the mechanism of curative effect.Methods（1）（early OA model） A total of 28 New Zealand white rabbits were randomly divided into A,B,C three groups.group A（treatment） and B（control） were both for 11 rabbits,and 6 rabbits were considered as blank control group（group C）.0.5 mL 1.6% papain was injected into the right knee joint cavity both group A and B according to three times（1,4,7 days）.The equivalent dilution was injected into the left knee joint cavity simultaneously.3 weeks after the first injection,and T₂ WI,3D-FS-SPGR,and T1ρ mapping in sagittal plain were scanned for three groups.Randomly selected two models pathology test in the group A and B,confirm early OA model is established.（2）（treat models） Then give epimedium lavage to group A two month.The equivalent sterile saline lavage to group B.A certain time（T0=0,T1=1 mon,T2=2 mon） after treated do MR scanned for group A and B.Analysis of the image.T1ρ values and the cartilage thickness in bilateral femoral condyle cartilage were measured by post-processing software.The all femurs were pathological examined.Tlp values and the cartilage thickness were statistic analysis.Results（1） Articular cavity injection of papain can successfully establish early osteoarthritis rabbit model which pathology has been confirmed.T1ρ values of the experimental（group A,B） were significant higher than of the control side（P<0.05）.Cartilage thickness values of the experimental（group A,B）and the control side has no statistical significance（P>0.05）.（2） T1ρ values of the Epimedium treat model（right side）were significant lower than of the pre-tre atment that of the preintervention（P<0.05）.T1ρ values of the NS treatment（left side） and the pre-treatment has no statistical significance（P>0.05）.Conclusion（1） T1ρ values of the early and advanced stage of OA had increased in various degree,and the values related cartilage matrix composition.（2） Single herb Epimedium has effective to treat early stages osteoarthritis in knee joint.Speculated that the possible mechanism for treatment common channel in the development of OA,which inhibition protease formation and promote proteoglycan secretion.更多还原