【摘要】 目的 评价功能MRI是否能反映肿瘤血管在抗肿瘤治疗前后的变化情况，并获得MRI相关半定量及定量参数。方法 构建结肠癌裸鼠BALB/cnu皮下移植瘤模型，共20只，用维吾尔医药（维药）小艾飞蜜膏对裸鼠进行治疗；监测移植瘤的微血管密度计数、VEGF和PCNA等参数，评价治疗效果和MRI监测结果。应用动态增强磁共振成像（DCEMRI）扫描，结合药代动力学模型，获得反映肿瘤细胞代谢及微血管通透性的各项半定量及定量参数，评估抗肿瘤治疗的早期疗效；根据药物的体外实验结果选择不同的观测点，分别随机处死1/2的载瘤裸鼠，进行免疫组化染色。将病理免疫组化染色结果与上述MRI各项定量、半定量参数进行相关性分析。结果 维药作用下的人结肠癌HT29细胞增殖率显著下降，比对照组降低了44.87%（P<0.05）。比对照组HT29细胞的凋亡率升高了2.45倍（P<0.05）。病理学检测与DCEMRI各参数的相关性结果显示：K^trans值和K_ep与VEGF、MVD和PCNA存在较好正相关（P<0.05）。结论 结肠癌裸鼠使用维药治疗前后不同时期DCEMRI检测获得的定量参数与免疫组化有较好的相关性。更多还原

【Abstract】 Objective To evaluate whether the functional MRI could be used to reflect the change of angiogenesis after drug treatment,and obtain the related semi quantitative and quantitative parameters by dynamic contrast-enhanced magnetic resonance imaging（DCE-MRI）.Methods The subcutaneous transplantation colorectal cancer were constructed in 20 nude mice,which were treated with Xiaoaifeimi,The transplanted tumor microvascular density,VEGF and PCNA were monitored.The therapeutic effect and MRI monitoring results were evaluated by combining DCE-MRI with the pharmacokinetic model.The semi quantitative and quantitative parameters were obtained for evaluating the effection of medicine.Half of nude mice were sacrificed to obtain the immunohistochemical staining.Correlation between pathological findings and parameters were analyzed.Results Compared to the control group,human colon HT-29 cell proliferation rate was significantly decreased（44.87%）（P<0.05）,and the apoptosis rate of HT-29 cells was increased 2.45 times（P<0.05） after Uyghur medicine treatment.The correlation between pathological examination and DCE-MRI parameters showed that K^trans value and K_ep were positively correlated with VEGF,MVD and PCNA（P<0.05）.Conclusion A good relationship is showed between immunohistochemistry and DCE-MRI quantitative parameters in Uygur drug treating human colon cancer mice.更多还原