【摘要】 目的 评估血浆相关基因甲基化在食管癌复发中的临床价值。方法 收集81例食管癌(ESCC)患者及60例健康体检者血浆。采用甲基化特异性PCR技术分析了81例食管癌患者及60例健康体检者血浆中四个基因SFRP-1,WIF-1,DKK-3和RUNX3启动子区域的甲基化。结果 这四种基因在食管癌血浆甲基化频率均超过25%,其中:SFRP-1为29.6%,WIF-1 35.8%,DKK-3 37.4%,RUNX-3 35.8%;健康体检者血浆中均未检测到相关基因的甲基化。食管癌患者血浆中SFRP-1(x~2==14.16,P<0.01),DKK-3(x~2==6.21,P<0.05)和RUNX3(x~2==11.75,P<0.01)基因甲基化明显与食管癌的复发有关,未发现WIF-1基因甲基化与食管癌复发有关(x~2=3.39,P>0.05)。结论食管癌血浆相关基因甲基化可能与食管癌复发相关。更多还原

【Abstract】 Objective To evaluate the clinical significance of promoter hypermethylation of esophageal squamous cell carcinoma(ESCC) in plasma.Methods Promoter hypermethylation status of 81 ESCC patients and 60 healthy persons were analyzed using a methylation marker panel in plasma with methylation-specific polymerase chain reaction and the association between promoter hypermethylation and the two-year recurrence of ESCC.Examined genes included SFRP-1,WIF-1,DKK-3and RUNX3.Results The frequencies of high-level methylation in ESCC plasma were at least 25%for the four genes:SFRP-1 29.6%,WIF-1 35.8%,DKK-3 37.4%and RUNX-3 35.8%.Hypermethylation status was not detected in plasma of healthy persons.Hypermethylation of SFRP-1(x~2 = 14.16,P<0.01).DKK-3(x~2 =6.21,P<0.05) and RUNX-3(x~2 =11.75,P<0.01) was significantly associated with an increased risk of ESCC recurrence.Conclnsion The status of promoter hypermethylation of examined genes in plasma may be associated with recurrence of ESCC.更多还原