【摘要】 目的 通过检测皮肤鳞癌组织及正常皮肤组织中E-cadlherin,p14ARF,P16^INK4a基因启动子甲基化的发生情况,探讨E-cadherin,p14ARF和P16^INK4a基因启动子甲基化在皮肤鳞癌发生发展中的作用。方法 采用酚-氯仿法提取30例皮肤鳞癌组织及正常皮肤组织的基因组DNA,应用甲基化特异性PCR对所提DNA进行E-cadherin,p14ARF和P16^INK4a基因甲基化检测。结果 癌组织中E-cadherin,p14ARF和P16^INK4a基因启动子甲基化的阳性率分别为36.7%（11/30）,60.0%（18/30）和53.3%（16/30）,而正常皮肤组织中相应的3个基因的甲基化率分别为10.0%（3/30）,6.7%（2/30）和6.7%（2/30）,均显著低于癌组织。结论 皮肤鳞癌中P16INK4a基因启动子甲基化与正常皮肤组织有明显差异,且在低分化癌中更多见;E-cadlherin基因启动子甲基化与正常皮肤比较有显著差异,并且有淋巴结转移多见的显著特征,皮肤鳞癌中p14ARF基因甲基化发生率明显高于正常皮肤,且与分化程度、淋巴结转移和临床分期有关系,同时p14ARF,E-Cadherin,p16^INK4a三种基因甲基化存在正相关关系,这3个基因的甲基化位点与皮肤鳞癌密切相关。更多还原

【Abstract】 Objective To discuss the function of E-cadherin,p14 ARF and p16^INK4a gene promoter methylution in the development of skin cancer by examining occurence conditions of E-cadherin,p14 ARF and P16^INK4a gene promoter methylution in the skin squamous carcinoma tissues and normal skin tissues.Methods Skin squamous cell carcinoma specimens were collected from 30 patients and normal skin tissues were collected from 30 individuals as controls.Promoter methylation was detected for E-cadherin,p14 ARF and P16^INK4a by methylation-specific PCR.Correlations between E-cadherin,pl4 ARF or P16I^INK4amethylation and clinicopathological parameters were analyzed by the Spearman rank test.Results Methylation of E-cadherin（36.7%）,p14ARF（60.0%） and P16^INK4a（53.3%） was significantly more common in skin squamous cell carcinoma than in normal skin tissue（10.0%,6.7%and 6.7%.respectively P<0.05）.Conclusion E-cadherin,p14 ARF and P16^INK4a methylation were positively correlated within skin squamous cell carcinoma.Furthermore,methylation of these gene promoters in skin squamous cell carcinoma was correlated with differentiation,lymph node metastasis,and clinical stage（P<0.05）.Aberrant methylation in promoters of E-cadherin,pl4 ARF and P16I^INK4a may promote occurrence and progression of skin squamous cell carcinoma.更多还原