Objective To analyze the mutation status of C-kit and platelet derived growth factor receptor A(PDGFRA)genes in gastrointestinal stromal tumor(GIST), and investigate the relationship between the mutation and the pathogenesis of GIST. Methods Genomic DNA was extracted from the paraffin sections of tumor tissues of 102 patients with GIST from Renmin Hosptital of Wuhan University and Zhongnan Hospital of Wuhan University, based on which the specific target gene fragments were amplified by PCR, identified by se...
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