Objective To construct recombinant adenovirus vector p Ad-4-h MASP-2 by using p Yr-adshuttle-4. Methods Human mannan-binding lectin associated serine protease-2(h MASP-2)gene was amplified by PCR, digested with Bam HⅡand Eco RⅡ, and inserted into plasmid p Yr-adshuttle-4. The constructed shuttle plasmid p Yr-ads-4-h MASP-2 and p Ad /PL-DEST adenovirus backbone vector was reconstructed through homologous recombination in mediation of LR enzyme.The obtained plasmid p Ad-4-h MASP-2 was transfected to HEK293 ce...
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