Objective To express the surface specific antigen VP1 of echovirus 6 in prokaryotic cells and prepare its polyclonal antibodies. Methods The fragments with strong antigenicity was screened from the amino acid sequence of VP1 protein, of which the sequences were optimized and inserted into expression vectors p GEX-4T-2 and p ET-28a(+)respectively. The constructed recombinant plasmids p GEX-4T-2-vp1 and p ET-28a-vp1 were identified by restriction analysis and sequencing, then transformed to E. coli BL21(DE3)f...
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