【摘要】 为得到玉米转录因子ABP9的准确基因组序列及其旁侧基因组序列,应用PCR法对玉米自交系齐319 BAC文库中含有ABP9基因的单克隆进行筛选,得到了19个一级阳性混池,从中选择BAC-103、BAC-159进一步筛选获得了4个阳性单克隆。以其中1个阳性单克隆BAC-103-C3为模板进行全序列测定,比对分析后将ABP9基因初定位于玉米自交系Mo17基因组的3号染色体上。另以玉米自交系齐319、自交系Mo17萌发种子的根尖染色体滴片为材料,以绿色荧光标记的ABP9基因ORF区质粒DNA为探针进行荧光原位杂交,确认在这2个自交系中ABP9基因定位于3号染色体上。ABP9基因组序列的获得及其染色体定位为该基因分子标记的开发及应用于分子育种奠定了基础。更多还原

【Abstract】 In order to obtain the accurate genomic sequence and its flanking genomic sequences of maize transcription factor ABP9,PCR method was used to screen the BAC library of maize inbred line Qi319 for identification of single BACs which contain ABP9. As a result,four positive single BACs were obtained from two randomly selected among 19 positive primary pools,namely BAC-103,BAC-159. By sequencing and sequence alignment of single clone BAC-103-C3 with Mo17 genome,ABP9 was mapped on chromosome 3 of Mo17. This result was confirmed by fluorescence in situ hybridization using green fluorescent-labeled ABP9 ORF as the probe with root tip chromosomes drops of germinating seeds of maize inbred lines Qi319 and Mo17. The knowledge of accurate genomic sequence and chromosomal localization of ABP9 will facilitate the development of molecular markers of this gene for use in molecular breeding.更多还原