【摘要】 在重组大肠杆菌中共表达了谷胱甘肽运输蛋白CydDC与双功能合成酶GshF,通过增强对谷胱甘肽的运输作用,提高谷胱甘肽产量。实验结果表明,目的蛋白CydDC及GshF均成功表达;重组菌MG1655(pTrc99a-as/p BAD33-cydDC)总谷胱甘肽产量为0.22 mmol/L,胞外谷胱甘肽含量显著增加,达到81.9%,分别是对照菌MG1655(pTrc99a-as/pBAD33)的1.11倍和1.29倍。更多还原

【Abstract】 This study is to co-express the CydDC and GshF in the recombinant Escherichia coli,and to increases the glutathioneproduction by enhancing glutathione transportation. As the results from the experiments,the target protein CydDC and Gsh F were successfullyexpressed,the glutathione production of MG1655(pTrc99a-as/pBAD33-cydDC)reached 0.22 mmol/L,and the content of glutathione insupernatant significantly increased and reached 81.9%,which was 1.11-fold and 1.29-fold of control strain MG1655(pTrc99a-as/p BAD33),respectively.更多还原