Construction of the prokaryotic expression system of yfiF gene in E. coli,optimization of its induced expression condition and purification of the protein
[Objective]To construct the prokaryotic expression system of yfi F gene,which is a function unknown gene of E. coli,to optimize its induced expression condition,and to purify the soluble Yfi F fusion protein. [Methods]Using p ET16 b expression plasmid to construct p ET16b- yfi F prokaryotic expression vector,then transformed it into E. coli BL21,and was induced by IPTG to express fusion protein Yfi F. The adding time,final concentration and induction time of IPTG was optimized. The soluble Yfi F fusion prot...
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
