[Objective]To construct the recombinant of rat Neurexin 1β( Nrx 1β) and its splice variant eukaryotic expression plasmid Nrx 1β(ΔS4),and to identify their expression ability in eukaryotic cells. [Methods]Target genes of Nrx 1β and Nrx1β(ΔS4) were expanded by RT- PCR using rat hippocampal total RNA as template. Then the target genes were sub- cloned into the p DsRed2- C1 vector by molecular biology method. The recombinants were transfected into HEK293 T cells,and protein expression was detected by Western Bl...
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