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水溶性蝇蛆甲壳素的提取及红外光谱分析

The Preparation of Water-Soluble Chitin from Fly Maggot and FTIR Analysis

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【作者】 李小波; 褚夫江; 沈娟; 金小宝;

【Author】 Li Xiao-bo;Chu Fu-jiang;Shen Juan;Jin Xiao-bao;Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances;School of Basic Courses, Guangdong Pharmaceutical University;

【机构】 广东省生物活性药物研究重点实验室; 广东药科大学基础学院;

【摘要】 甲壳素是药用昆虫蝇蛆的生物活性成分之一,本研究采用酸碱法并辅以微波加热提取蝇蛆甲壳素,继而以碱冻融法制备低脱乙酰度的水溶性甲壳素。FTIR光谱分析结果表明,2种样品均具有3个典型的甲壳素酰胺谱带特征吸收峰,分别为1653cm-1和1 621cm-1(Ⅰ)、1 555cm-1(Ⅱ)、1 309cm-1(Ⅲ)(蝇蛆甲壳素初提物),以及1 625cm-1(Ⅰ)、1 524cm-1(Ⅱ)和1 305cm-1(Ⅲ)(水溶性蝇蛆甲壳素)。家蝇蛆水溶性甲壳素的制备有助于拓展其深加式利用。

【Abstract】 Chitin is one of the bioactive components of medicinal insect maggots. In this study, fly maggot chitin was extracted by acid-alkali method, assisted by microwave heating, and water-soluble chitin with low deacetylation degree was further prepared by alkali-freezing-thawing treatment. FTIR spectral analysis results showed that the two samples had three typical absorption peaks of amide bands of chitin in both samples: 1 653 cm-1 & 1 621 ㎝-1( Ⅰ), 1 555cm-1( Ⅱ) and 1 309 cm-1( Ⅲ), for initial extraction of maggot chitin; 1 625 cm-1( Ⅰ), 1 524 cm-1( Ⅱ), 1 305 cm-1( Ⅲ), for further preparation of water-soluble chitin, respectively. The preparation of water-soluble chitin can be beneficial for the deep development and utilization of medicinal maggots.

【关键词】 蝇蛆; 水溶性甲壳素; 傅里叶红外光谱;
【Key words】 Fly-maggot; Water soluble chitin; FTIR;
【基金】 2015年广东省医学科学技术研究基金项目(No.A2015040)
  • 【文献出处】 生物化工 ,Biological Chemical Engineering , 编辑部邮箱 ,2016年06期
  • 【分类号】TQ464;O657.33
  • 【被引频次】2
  • 【下载频次】210
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