【摘要】 转录因子Rex是一种广泛存在于革兰氏阳性菌,能够与NADH或者NAD~+直接结合响应胞内NADH/NAD~+的氧化还原传感器,与靶基因的结合可调节细胞内的多种生理代谢。NAD(H)是调节细胞能量代谢的必需辅酶,显示微生物细胞内的氧化还原状态。研究发现Rex的调节活性与细胞内NADH/NAD~+比率相关。需氧和厌氧菌属中Rex单体和复合物晶体结构的解析揭示了Rex、NADH/NAD~+和靶基因间的作用关系及调控机制。通过比较分析了不同菌株中Rex单体和复合物的晶体蛋白结构,并揭示了NADH/NAD~+对Rex调控活性的影响,进一步解析了Rex与碳和能量代谢、厌氧代谢、发酵、生物膜等之间的联系,并展望了Rex的研究和应用方向。更多还原

【Abstract】 Transcription factor Rex is a sensor of intracellular NADH / NAD~+ redox state by incorporating either NADH or NAD+in Gram-positive bacteria. In response to NADH / NAD~+ ratio,Rex could control many physiological metabolisms by regulating genes expression. NAD [H ], the key coenzyme in the metabolic network,could reflect the cellular redox state and influence the activity of Rex. The structures of Rex,either alone or bound with cofactor and / or DNA operator,have been determined in aerobic and anaerobic group. The regulatory mechanism of Rex and the relationship of Rex,NADH / NAD~+,and target genes were revealed through comparative analysis on its structure in aerobic and anaerobic bacterial. Meanwhile,the connections of Rex with central carbon,energy metabolism,anaerobic metabolism,fermentation,biofilm formation,oxidative stress as well as cell toxicity were elucidated. In addition,further study trends and applications of Rex were discussed.更多还原