【摘要】 目的:探究低强度振动载荷刺激对于三维多孔钛合金(porous titanium,pTi)内兔成骨细胞(osteoblasts,OB)粘附和增殖的影响,以期为临床振动载荷协同pTi治疗骨缺损提供重要的实验依据。方法:原代分离培养1日龄新生新西兰兔颅盖骨OB,待传至第3~6代以5×104/mL密度植入pTi中,随机分为振动组和对照组。振动组在5%CO2、37℃温度环境下进行3天低强度振动载荷(0.5 g,30 Hz)刺激,每天1 h;对照组放置在无载荷的振动平台上,分别采用DAPI核染色法和MTT法评价OB在p Ti中的粘附和增殖情况。结果:在振动载荷刺激下,每个视野下OB细胞在pTi上的粘附数量14±3个,对照组粘附数量6±2个,粘附能力显著增强(P<0.05);在振动载荷刺激下,pTi上的OB细胞增殖吸光度为36.5%±0.8%,对照组吸光度为34%±1%,细胞增殖能力得到了显著促进(P<0.05)。结论:低强度振动载荷刺激对于提高pTi中的OB细胞生物学活性具有促进作用,本研究为下一步系统探索振动载荷协同pTi对于骨缺损的修复效果奠定了基础。更多还原

【Abstract】 Objective: To determine the effect of low-level mechanical vibration on proliferation and attachment of osteoblasts in the 3-D porous titanium implants(p Ti), and thus provide critical experimental basis for combinative application of mechanical vibration and pTi in the treatment of osseous defects in clinics. Methods: Primary osteoblasts(OB) were obtained by digesting the calvarial bone of 1-day-old New Zealand rabbits. Cells with passages 3~6 were used in the experiment. Primary osteoblasts were seeded onto the pTi implants at a density of 5×10~4cells/mL, and random Ly divided into the control and mechanical vibration group. Cells in the mechanical vibration group were subjected to 1 h/day mechanical vibratory stimulation(0.5 g, 30 Hz) for 3 days at room temperature. Cells in the control group were placed onto the inactivated mechanical loading platform. MTT assays were performed to determine the cell proliferation and DAPI assays were performed to evaluate the cell attachment in pTi. Results: The DAPI staining results demonstrate that the osteoblastic attachment number in pTi in the mechanical vibration group was significantly higher than the control group(P<0.05).MTT assays show that the mechanical vibration group showed increased cell proliferation as compared with the control group.Conclusions: Our present study shows that mechanical vibration improved the biological activities of osteoblasts in pTi, which lays the foundation of the systemic investigation of pTi combined with mechanical vibration in bone defect repair.更多还原