【摘要】 目的:探讨骨肉瘤组织中Wnt5a、Ror2表达。方法:免疫组织化学法检测Wnt5a、Ror2在实验组55例骨肉瘤和对照组15例骨软骨瘤组织标本中的表达。结果:Wnt5a、Ror2在骨肉瘤组的表达均明显高于骨软骨瘤组(Wnt5a:74.55%、20.00%,Ror2:13.33%、70.91%,P<0.05)。Wnt5a、Ror2在骨肉瘤出现转移的表达明显高于未转移(Wnt5a:100%、66.67%,Ror2:100%、61.90%,P<0.05),在骨肉瘤Enneking分期中,Wnt5aⅠ期11.11%,Ⅱ期84.38%,Ⅲ期100.00%,Ror2Ⅰ期22.22%,Ⅱ期72.73%,Ⅲ期100.00%,Ⅰ期与Ⅱ期间和Ⅰ期与Ⅲ期间差异皆有统计学意义(P<0.05),而Ⅱ期与Ⅲ期间差异皆无统计学意义(P>0.05),Wnt5a、Ror2两者之间的表达呈正相关分布(骨肉瘤组:r=0.844,P<0.01;骨软骨瘤组:r=0.808,P<0.01),但在骨肉瘤患者性别、年龄以及骨肉瘤病理分型组中表达无明显差异(P>0.05)。结论:Wnt5a、Ror2在骨肉瘤中存在高度表达,可能具有促癌作用,并与其恶性程度和侵袭转移有关,二者协同作用。更多还原

【Abstract】 To investigate the expression and clinical significance of Wnt5 a and Ror2 in human osteosarcoma tissues.Methods: SP immunohistochemistry was performed to detect the expressions of Wnt5 a and Ror2 protein in specimens of 55 osteosarcoma patients and 15 osteochondroma patients. Then the interrelation of the expressions of wnt5 a and Ror2 were calculated and analyzed. Results: The positive rate of both Wnt5 a and Ror2 expression showed significant differences between osteosarcoma group and osteochondroma group(Wnt5a: 74.55%, 20.00%, Ror2: 13.33%, 70.91%, P<0.05), and showed significant differences between osteosarcoma metastasis group and non-metastasis group(Wnt5a: 100 %, 66.67 %; Ror2: 100 %, 61.90 %, P<0.05). In osteosarcoma Enneking stage, the expression of Wnt5 a in I, Ⅱ and Ⅲ stage was11.11%, 84.38 %, Ⅲ 100.00%, respectively; the expression of Ror2 in I, Ⅱ and Ⅲ stage was 22.22 %, Ⅱ 72.73 %, 100.00 %, respectively. The positive rate of Wnt5 a was similar to Ror2, and both of them were statistically significant between Enneking stage Ⅰ and Ⅱ, Ⅰ and Ⅲ(P<0.05), but no significance between Enneking stage Ⅱ andⅢ(P>0.05). A positive correlation existed between the expressions of wnt5 a and Ror2(osteosarcoma: r=0.844, P<0.01; osteochondroma:r=0.808, P<0.01), but the differences of the positive rate of both Wnt5 a and Ror2 in osteosarcoma sex, age and pathological type were not statistically significant(P>0.05). Conclusions: Wnt5 a and Ror2 may act as a tumor promoter in osteosarcoma with synergistic effect,which are positively related to the severity, invasion and metastasis of osteosarcoma.更多还原