【摘要】 以氮掺杂碳球修饰玻碳电极（N-Cs/GCE）为工作电极,采用交联法固定氯化乙酰胆碱酯酶（ACh E）,制备了ACh E/N-Cs/GCE传感器,对有机磷农药敌敌畏进行检测分析。结果表明:在(6.5×10^-116.5×10^-6)g/L范围内,敌敌畏抑制率与其浓度负对数呈良好的线性关系,线性方程为Y（%）=62.488-5.149X（X为敌敌畏浓度负对数）,R²=0.993,相关性较好。按抑制率10%计算,检出限为6.46×10^-11 g/L。用该方法对苹果皮中敌敌畏进行检测,回收率在89.32%⁹3.55%范围内,回收效果较好,准确度较高,具有较强的实用价值。更多还原

【Abstract】 Glassy carbon electrode（GCE） modified with nitrogen-doped carbon spheres（N-Cs） and fixed acetylcholinesterase（ACh E） was employed as working electrode to fabricate the ACh E/N-Cs/GCE biosensor for detecting dichlorvos. The results show that there was a wide linear from 6.5×10^-11 to 6.5×10^-6g/L between the inhibition rate of dichlorvos and the negative logarithm of concentration of dichlorvos, the equation was Y（%）=62.488-5.149X（X is the negative logarithm of concentration of dichlorvos）, R²=0.993, Calculated by inhibition rate 10%, the detection limit was 6.46×10^-11 g/L. The sensor could be used for detection dichlorvos in the apple’s peel, with recoveries of 89.32%⁹3.55%, recycling is better and the accuracy of the method was very high, which has a strong practical value.更多还原