【摘要】 目的探讨慢性阻塞性肺疾病(chronic obstructive plumonary disease,COPD)患者急性加重期治疗前后血清高迁移率族蛋白B1(high mobility group protein B1,HMGB1)的水平变化及其与肺功能的相关性。方法选取2014年10月—2015年6月COPD急性加重期患者72例,依据肺功能将其分为两亚组:GOLD 1、2级患者36例,GOLD 3级患者36例,另选取对照组36例,采用ELISA法分别测定所有对象的血清HMGB1水平,并行肺功能检测,计量资料比较采用t检验,相关关系采用Pearson相关性分析,P<0.05为差异有统计学意义。结果 COPD急性加重患者治疗前及治疗后血清HMGB1表达水平均明显高于对照组[(5.47±1.69)、(3.41±0.99)、(1.95±0.16)μg/L],对比差异均有统计学意义(均P<0.05)。GOLD 3级COPD患者治疗前及治疗后血清HMGB1表达水平明显高于GOLD 1、2级COPD患者治疗前及治疗后[(6.84±1.21)、(4.11±0.70)、(4.17±0.85)、(2.66±0.32)μg/L],对比差异均有统计学意义(均P<0.05)。COPD患者血清HMGB1水平与FEV1%pred呈显著负相关(r=-0.749,P<0.05)。结论血清HMGB1在COPD患者中表达水平明显升高,且与病情严重程度呈显著相关,提示检测血清HMGB1对COPD病情严重程度的判断有一定意义。更多还原

【Abstract】 Objective To explore the changes and correlation between high mobility group protein B1(HMGB1) and lung function of serum levels of HMGB1 in the patients with chronic obstructive pulmonary disease(COPD) before and after treatment.Methods 72 cases patients with acute exacerbation of COPD,according to the pulmonary function were divided into two subgroups from October 2014 to June 2015,GOLD 1,2 grade patients with 36 cases,GOLD 3 grade patients with 36 cases,then control group with 36 cases,to detect the serum levels of HMGB1 in acute exacerbation of COPD before treatment,after treatment of acute exacerbation of COPD and healthy subjects(control group),which were measured by enzyme- linked immunosorbent assay(ELISA),and pulmonary function test was performed,measurement data was processed by t test,the result of P<0.05 was considered statistically significant.Results COPD expression level of HMGB1 in patients with acute exacerbation of patients before and after treatment were higher than those of the control group [(5.47 ±1.69),(3.41 ±0.99),(1.95 ±0.16)μg/L],the differences were statistically significant(all P<0.05).Serum levels of HMGB1 in GOLD 3 grade COPD before and after treatment were higher than that in GOLD 1,2 grade COPD [(6.84±1.21),(4.11±0.70),(4.17±0.85),(2.66±0.32)μg/L],the differences were statistically significant(all P<0.05).Serum levels of HMGB1 were significant negative correlated with FEV1% pred(r=- 0.749,P<0.05).Conclusion The expression level of serum HMGB1 in patients with COPD was significantly increased,and it was significantly correlated with the severity of the disease.It was suggested that the detection of serum HMGB1 had a certain significance with the severity of COPD.更多还原