【摘要】 建立了正相高效液相色谱测定食物中8种维生素E异构体及维生素A的方法。样品中的维生素E异构体和维生素A经皂化和液液萃取,Waters ACQUITYTMUPLC BEH Amide色谱柱(150 mm×3.0 mm,1.7μm)分离,体积比为90∶10的正己烷与叔丁基甲基醚-四氢呋喃-甲醇(20∶10∶1,v/v/v)为流动相,荧光检测器和紫外检测器串联检测。4种生育酚在5.0~60.0 mg/L(r~2≥0.999 9)、维生素A和4种三烯生育酚在0.5~6.0 mg/L(r~2≥0.999 6)范围内具有良好的线性,各基质中目标物的检出限在20~60μg/kg之间;9个组分在各基质中的加标回收率为79.2%~114.2%,相对标准偏差(RSD)为1%~12%。该方法简便、灵敏、可靠、环保,可用于食物中8种维生素E异构体以及维生素A含量的同时测定。更多还原

【Abstract】 A synchronous detection method was established by normal-phase(NP)-HPLC to separate and determine eight vitamin E isomers and vitamin A in foods. The sample was pretreated and then separated on a Waters ACQUITYTMUPLC BEH Amide column(150 mm × 3. 0mm,1. 7 μm). The mobile phase consisted of hexane(A,90%)and tert-butyl methyl ethertetrahydrofuran-methanol(20 ∶10 ∶1,v/v/v)(B,10%). The analytes were detected by UV and fluorescence detectors,identified and quantified with external standards. The linear ranges of tocopherols were from 5. 0 mg/L to 60. 0 mg/L(r2≥0. 999 9),and those of tocotrienols and vitamin A were from 0.5 mg/L to 6.0 mg/L(r2≥0. 999 6),while the LODs of vitamin E isomers and vitamin A were from 20 μg/kg to 60 μg/kg. The method is of high precision,stability and repeatability,and the recoveries were from 79. 2% to 114. 2%. This method is simple,accurate and suitable for the analysis of vitamin E isomers and vitamin A in foods.更多还原