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蜂蜜中蛋白质及氨基酸含量的测定
The Content Determination of Protein and Amino acid in Honey
【摘要】 分别采用考马斯亮蓝法和茚三酮显色法测定6个蜂蜜样品中的蛋白质和氨基酸含量。实验结果表明,100 g蜂蜜样品中,蛋白质和氨基酸的含量分别为0.044~0.391 g和0.031~0.055 g,表明不同蜂蜜样品中的蛋白质和氨基酸的含量各不相同。蜂蜜样品中蛋白质和氨基酸测定时的相对标准偏差分别为0.666%~6.447%和0.601%~1.306%,平行样品间的测定误差较小。
【Abstract】 The methods pf Bradford and Ninhydrin colorimetric were chosen to determine the contents of protein and amino acid of six samples of honeys. Results showed that the contents of protein and amino acid in 100 g honey samples were 0.044 ~ 0.391 g and 0.031 ~ 0.055 g, the contents of them in six honey samples were different from each other. The relative standard deviations was 0.666% ~ 6.447% for protein test and 0.601% ~ 1.306% for amino acid test. All deviations among the parallel samples were small.
【关键词】 蜂蜜;
氨基酸;
蛋白质;
茚三酮;
考马斯亮蓝;
【Key words】 Honey; Amino acids; Proteins; Ninhydrin; Coomassie brilliant blue;
【Key words】 Honey; Amino acids; Proteins; Ninhydrin; Coomassie brilliant blue;
- 【文献出处】 现代食品 ,Modern Food , 编辑部邮箱 ,2016年20期
- 【分类号】S896.1
- 【被引频次】15
- 【下载频次】773