This work aimed to develop an effective novel method to synthesize artificial antigens of forchlorfenuron(CPPU).Towards this goal,Friedel-Crafts reaction was used to modify the structure of forchlorfenuron with the catalysis of anhydrous aluminum chloride and the hapten was coupled with bovine serum albumin(BSA) and ovalbumin(OVA)by carbondiimine(EDC) method,respectively,to obtain forchlorfenuron immunogen and coating antigen.The molecular structure of the forchlorfenuron hapten was identified by elemental ...
引文格式:肖石妹,鄢爱平,肖芳,等.氯吡脲人工抗原的合成与鉴定[J].食品科学,2016,37(21):183-188.DOI:10.7506/spkx1002-6630-201621031.http://www.spkx.net.cnXIAO Shimei,YAN Aiping,XIAO Fang,et al.Synthesis and identification of artificial antigens of forchlorfenur