应用制备型高速逆流色谱法(HSCCC)分离纯化艾叶中的异绿原酸A。选用弱极性溶剂体系正己烷-乙酸乙酯-乙醇-水(3∶7∶4∶6,V/V)为两相溶剂系统,上相为固定相,下相为流动相,在流速2 m L/min,转速900 r/min,检测波长280 nm的条件下对粗提物进行制备分离,一次进样25 mg,可得到11 mg样品。利用高效液相色谱(HPLC)检测样品的纯度为99.01%。将得到的样品进行高效液相-离子阱-飞行时间质谱(LCMS-IT-TOF)、核磁共振氢谱(1H-NMR)检测确定其化学结构,并确定该样品为异绿原酸A。实验表明制备型高速逆流色谱可以成功地将艾叶中的异绿原酸A进行分离纯化,该方法分离效率高,对异绿原酸A在食品医疗领域的应用具有重要意义。
【英文摘要】
Isochlorogenic acid A was separated and purified from Artemisiae argyi folium by preparative high-speed counter- current chromatography( HSCCC) using a weak polarity solvent system consisting of n- hexaneethyl acetate- ethanol- water( 3∶ 7∶ 4∶ 6,v / v / v / v),the upper phase acted as stationary phase and the lower phase served as mobile phase.The separation process was performed at a flow rate of 2 m L / min,the rotation rate of the apparatus was set at 900 r / min,and the effluents were detected at 280 nm...
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