【摘要】 本研究制备了一种以硅胶为基材,表面接枝4,4’-二羟基查耳酮的新型交联壳聚糖材料(D-Chitosan@Si),用于选择性分离和富集天然植物中黄酮类化合物,并以槲皮素为吸附对象,考察了影响D-Chitosan@Si吸附性能的因素,分析其吸附机制。研究显示,D-Chitosan@Si对槲皮素的吸附为吸热过程,符合Langmuir吸附模型;槲皮素在DChitosan@Si上的吸附可能受多个步骤共同控制,并且准二级吸附动力学方程能更好地描述槲皮素在D-Chitosan@Si上的吸附行为。进一步将D-Chitsoan@Si用于传统中药款冬花中黄酮类化合物的分离纯化,总黄酮的吸附量可达52.31 mg/g,处理后的款冬花总黄酮的纯度由18.9%提高到85.6%,D-Chitosan@Si表现出较好的选择性吸附效果。更多还原

【Abstract】 A silica based cross linking chitosan grafted with 4, 4 ’-dihydroxychalcone( D-Chitosan@Si) was designed and prepared for selective separation and enrichment of flavonoids. The factors which affect the adsorption capacity of D-Chitosan@Si and the absorption mechanism were discussed in detail. The experimental results showed that the adsorption of Quercetin on D-Chitosan@Si was described by the Langmuir model,and the adsorption followed the pseudo second order kinetics equation,was controlled by multiple. D-Chitosan@Si was also successfully used to separate the flavonoids of tussilago farfara,the absorptive capacitythe of flavonoids was 52.31 mg/g and the purity was increased from 18.9% to 85.6%. It indicated that D-Chitosan@Si had high selectivity on enrichment flavonoids.更多还原