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纳米氧化锌对甜瓜主要致病菌抑菌活性研究

The inhibitory effects of zinc oxide nanoparticles treatment on the important pathogenic fungi causing postharvest disease in muskmelon

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【作者】 王虎军路军薛华丽赵军毕阳南米娜

【Author】 WANG Hu-jun;LU jun;XUE Hua-li;ZHAO Jun;BI Yang;NAN Mi-na;College of Science,Gansu Agricultural University;Institute of Agricultural Resources Chemistry and Application,Gansu Agricultural University;College of Food Science and Engineering,Gansu Agricultural University;

【机构】 甘肃农业大学理学院甘肃农业大学农业资源化学与应用研究所甘肃农业大学食品科学与工程学院

【摘要】 为研究纳米氧化锌对引起采后甜瓜粉霉病、白霉病和黑斑病的主要致病菌粉红单端孢(Trichothecium roseum)、镰刀菌(Fusarium spp.)、交链孢(Alternaria)的抑制效果,本文通过不同浓度的固相和液相纳米氧化锌对粉红单端孢(T.roseum)、硫色镰刀菌(Fusarium sulphureum)、互隔交链孢(Alternaria alternate)三种菌分别进行体外、体内抑菌活性实验研究。体外实验结果表明:固相和液相纳米氧化锌对这三种病原菌菌丝生长、孢子萌发均具有明显的抑制作用,且随着溶液浓度的增大,抑制效果增强;固相纳米氧化锌较液相具有更佳的抑菌效果,且对T.roseum的抑制作用最强,其次是F.sulphureum、A.alternate。体内实验结果表明,固相和液相纳米氧化锌处理均可显著抑制由T.roseum、F.sulphureum、A.alternate损伤接种甜瓜果实病斑直径的扩展。故两种性状纳米氧化锌均可通过抑制甜瓜采后主要致病菌的生长来减轻甜瓜采后病害,且固相纳米氧化锌较液相纳米氧化锌效果更为明显。

【Abstract】 To study the inhibitory effects of the zinc oxide nanoparticles on T. roseum,Fusarium spp.,Alternaria,which caused pink rot,Fusarium rot,black spot of postharvest muskmelon,respectively. The solid phase and liquid phase zinc oxide nanoparticles at different concentration and T. roseum,F. Sulphureum,A. alternate were used in vitro and vivo study. In vitro,the results showed that the significant inhibitory effect on mycelial growth and spore germination was found at different states of zinc oxide nanoparticles. The inhibition effect was greatly enhanced with the increase of the zinc oxide nanoparticles’ s concentration. The inhibitory effect of the solid phase zinc oxide nanoparticles was more effective than the liquid phase one,which showed the most effect for T. roseum,then F. sulphureum,A. alternate. In vivo,it indicated that the solid phase and liquid phase zinc oxide nanoparticles had the significant inhibitory effect on the diameter of melon disease spot,which was caused by T. roseum,F. sulphureum,A. alternate,respectively. So zinc oxide nanoparticles could directly inhibit the major of pathogens to decrease disease of postharvest muskmelon,besides the inhibitory effect of solid phase zinc oxide nanoparticles was better than the liquid.

【基金】 公益性行业(农业)科研专项(201303075);甘肃省高等学校科研项目和甘肃农业大学伏羲人才青年基金项目
  • 【文献出处】 食品工业科技 ,Science and Technology of Food Industry , 编辑部邮箱 ,2016年04期
  • 【分类号】TS255.1
  • 【被引频次】12
  • 【下载频次】369
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