【摘要】 利用普通硅胶柱,选用乙醚—正己烷作流动相对姜油树脂进行初步分离,采用GC—MS法进行跟踪检测。对比不同展开剂下β-倍半水芹烯和姜烯混合液在硝酸银硅胶板上的层析情况,选用体积比为991的正己烷—乙酸乙酯流动相体系分离其中的β-倍半水芹烯。结果表明:β-倍半水芹烯的GC—MS峰面积百分数达95.79%,收率为89.73%。通过对β-倍半水芹烯峰值样进行HPLC检测,证明β-倍半水芹烯的紫外特征吸收波长为232nm,在姜烯的特征吸收峰262nm下基本无吸收。更多还原

【Abstract】 A aether and n-hexane solution was used as mobile phase to preliminary extractβ-sesquiphellandrene from ginger oleoresin by common silica gel column chromatography,and GC—MS was used for detectingβ-sesquiphellandrene tracing.Comparison of different developing solvents ofβ-sesquiphellandrene and zingiberene on argentated silica gel G pre-coated plate,hexane-ethyl acetate(991)was used as the mobile phase to sepatateβ-sesquiphellandrene,the results indicated that the GC—MS area percentage of the silica gel and argentation column chromatography product was 95.79% with 89.73% recovery.Through the HPLC detection ofβ-sesquiphellandrene peak sample,β-sesquiphellandrene was found its existence of UV absorption at 232 nm,and generally had no absorption at 262 nm the characteristic absorption of zingiberene.更多还原