【摘要】 为探究提取葛根中葛根素的最佳工艺条件,以葛根素浓度为响应值,利用单因素试验和响应面设计对葛根素的醇提工艺进行优化,并采用高效液相色谱法(HLPC)对最佳工艺条件下所得提取物进行鉴定。结果表明:葛根素的最佳提取条件为乙醇体积分数50%、料液比125(mV)、提取时间70min、提取温度60℃,该条件下所得葛根素为预测值的96.79%,HPLC检测结果与葛根素标准品一致。更多还原

【Abstract】 In order to explore the optimum conditions of puerarin,the extraction process was optimized by single factor test and response surface design.The extract was identified by high performance liquid chromatography(HLPC)for puerarin or not.The results showed that the best extraction conditions were:ethanol volume fraction50%,solid-liquid ratio of 125(mV),extraction time 70 min and extraction temperature of 60 ℃With those conditions,the expected content of puerarin is 96.79%,and the testing results of HPLC is consistent with the element standard of puerarin.更多还原