酵母的生产效率和成本在很大程度上依赖于培养模式。根据酵母的生长特性,提出了一种新的线性减小比生长速率指数补料(exponential-feeding based on linear reducing specific-growth-rate,LR-μ-EF)策略。首先,采用该策略进行抗冻酵母Saccharomyces cerevisiae AFY-1的3 L发酵罐培养试验,比较了培养过程中生物量的计算值和实验值。然后在此基础上,以最终生物量、收率和生产速度为酵母生产效率的评价指标,讨论了补料速度和累计补料体积对酵母生产效率的影响。当恒定比生长速率指数补料(constant-μexponential-feeding,C-μ-EF)培养阶段的比生长速率(μ)为0.05 h-1、LR-μ-EF培养阶段的μ减小速度为0.000 8 h-2时,生物量的实验值与计算值基本一致。在累计补料体积560 m L的条件下,最终生物量和酵母收率随着μ减小速度的增大而逐渐上升,当μ减小速度为0.000 8 h-2时分别达到77.5g/L和38.0%。这与μ减小速度为0(即C-μ-EF策略)时相比,分别增加了26.7%和27.5%。此...
【英文摘要】
The production efficiency and cost of yeasts depend greatly on culture modes. A novel strategy for exponential feeding based on linear reducing specific-growth-rate( LR-μ-EF strategy) was proposed according to their growth characteristics. Fed-batch culture of freeze-tolerant yeast Saccharomyces cerevisiae AFY-1 was carried out in a3 L stirred tank bioreactor with the feeding strategy. The experimental values of biomass during culture were compared with calculated values. Then,the effects of feeding rate an...
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