Myocytes in the pulmonary veins(PV) play a pivotal role in the development of paroxysmal atrial fibrillation(AF). It is therefore important to understand physiological characteristics of these cells. Studies on these cells are,however,markedly impeded by the fact that single PV myocytes are very difficult to obtain due to lack of effective isolation methods. In this study,we described a novel PV myocyte isolation method. The key aspect of this method is to establish a combination of retrograde heart perfusi...
【基金】
supported by the National Natural Science Foundation of China(No.30670854,81100818);
the Fundamental Research Funds for the Central Universities,Huazhong University of Science and Technology,China(No.01-09-510856,01-09-510812,01-09-510816,01-18-510038,01-04-510163)
【更新日期】
2016-03-29
【分类号】
R33
【正文快照】
Atrial fibrillation(AF)is the most common sustainedcardiac arrhythmia and is associated with impairedquality of life and increased risk of stroke and mortali-ty[1].Even though the underlying mechanism of AF isstill not clear,the importance of the pulmonar
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