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HPLC法测定杜仲茶中车叶草苷含量的研究

Study on HPLC Method for Asperuloside Detecting in Eucommiae Tea

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【作者】 马伟曾里程健郭艳胡坚冉旭曾凡骏

【Author】 MA Wei;ZENG Li;CHENG Jian;GUO Yan;HU Jian;RAN Xu;ZENG Fan-jun;Institute of Textiles and Food,Sichuan University;Guangdu Pharmaceutical Health Products Co.,Ltd.;Sichuan Center for Disease Control and Prevention;

【机构】 四川大学轻纺与食品学院四川广都医药保健品有限责任公司四川省疾病预防控制中心

【摘要】 采用C18柱,流动相为甲醇+水+磷酸(66+870+1),流速1.5m L/min,检测波长237nm,柱温40℃左右,进样量20μL。通过研究检测线性关系、稳定性、精密度、重复性、加标回收率等,建立了HPLC法测定杜仲茶中车叶草苷含量的检测方法。结果表明:杜仲茶车叶草苷在0.0126-0.1134mg·mL-1之间线性良好(R2=0.9993),平均回收率为102.9%,测得车叶草苷含量为1.48%。该方法准确性高,重现性好,是一种快速、简便检测杜仲茶中车叶草苷含量的方法。

【Abstract】 A C18 column(Mightysil RP-18 GP Aqua 150-4.65μm) was adopted as stationary phase, mobile phase of methanol+water+phosphate(66+870+1), the flow rate of 1.5m L/min, detection wavelength of 237 nm, column temperature 40℃, 20μL of incoming sample amount. By studying the detection of linear relation, stability, precision,reproducibility, standard addition recovery rate, etc., established a HPLC method for determination of asperuloside from eucommia tea. Results show that eucommia tea asperuloside content had a good linear relation(R~2=0.9993) in the range of 0.0126-0.1134mg/mL, the average recovery was 102.9%, asperuloside content is 1.48%. The method is of high accuracy, good reproducibility and it is a fast and simple method of testing asperuloside content of Eucommia tea.

【关键词】 杜仲茶车叶草苷HPLC
【Key words】 eucommia teaasperulosideHPLC
  • 【文献出处】 食品与发酵科技 ,Food and Fermentation Technology , 编辑部邮箱 ,2016年03期
  • 【分类号】TS272.7;O657.72
  • 【被引频次】3
  • 【下载频次】173
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