Objective To explore the impacts of eucommia on the proliferation of bone mesenchymal stem cells( BMSCs) and neuronal differentiation. Methods BMSCs were isolated from tibia and femur of adult mice and cultivated in DMEM medium containing 10% FBS. Adding eucommia( 10 mg / ml) into the medium containing the 4th generation of BMSCs,in 7 days,the cell proliferation was observed under phase contrast microscope and determined by MTT method. In 28 days,the cell differentiation was determined by immunofluorescent ...
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