【摘要】 目的:观察大麻素对背根节神经元ATP诱发的[Ca²⁺]i升高的影响及机制。方法:培养SD大鼠背根节神经元,采用激光共聚焦技术检测培养神经元[Ca²⁺]i的变化。结果:ATP（100μmol/L）经P2X受体介导可导致培养的背根节神经元[Ca²⁺]i增高（P<0.05）;大麻素受体激动剂CP55940预孵育10 min可剂量依赖性地抑制背根节神经元ATP所致的[Ca²⁺]i升高（P<0.05）;CB1受体（cannabinoid receptor 1,B1R）的拮抗剂AM251（10μmol/L）、CB2受体（Cannabinoid receptor 2,CB2R）的拮抗剂AM630（10μmol/L）均可显著降低CP55940（1μmol/L）的抑制效应（P<0.05）;腺苷酸环化酶激动剂Forskolin（10μmol/L）可逆转CP55940对ATP的抑制作用（P<0.05）。结论:CP55940可显著抑制背根节神经元ATP诱发的[Ca²⁺]i升高,CP55940的抑制效应可能是由CB1、CB2受体介导抑制背根节神经元PKA活性所致。更多还原

【Abstract】 Objective: To explore whether cannabinoid has an effect on ATP-induced intracellular calcium concentration [Ca²⁺]i increase in cultured dorsal root ganglion neurons and the related mechanism. Methods: Neurons from SD rat dorsal root ganglion were cultured. Changes of intracellular calcium fluorescence intensity in cultured DRG neurons were measured by laser scanning confocal microscopy. Results: ATP（ 100 μmol / L） caused the increase of [Ca²⁺]i in DRG neurons via activation of P2 X receptors（ P < 0. 05）. A 10 min incubation with CP55940,the agonist of cannabinoid receptor,inhibited ATP-induced [Ca²⁺]i increase in DRG neurons in a concentration-dependent manner. The inhibitory effect of CP55940 on ATP-induced [Ca²⁺]i increase was blocked by CB1receptor（ Cannabinoid receptor 1,CB1R）antagonist AM251（ 10 μmol / L） and CB2receptor（ Cannabinoid receptor 2,CB2R） antagonist AM630（ 10 μmol / L）,respectively（ P < 0. 05）. Forskolin（ adenosine cyclase agonist,10 μmol / L） markedly suppressed the inhibitory effect of CP55940（ P < 0. 05）. Conclusion: CP55940 can inhibit ATP-induced [Ca²⁺]i increase in DRG neurons. The inhibitory effect of CP55940 may be mediated by CB1 and CB2receptors,which block PKA activity of DRG neurons.更多还原