To investigate whether the mi RNA-200 b can target GNAQ genes and regulate the estrous process. The Dual-luciferase vectors was applied to test the interfering effect of mi RNA-200 b on GNAQ genes, and the expression of mi R-200 b gene in hypothalamus nerve cells and ovarian granulosa cells was measured. The GNAQ downstream gene expression was measured by Q-PCR. The results showed that the value of Dual-luciferaseon of the GNAQ-3'UTR-psi-CHECKTM-2 and pri-mi R-200b-pc DNA3.1(+) transfection group was 77.5% ...
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
