【摘要】 目的 经颅注射无水乙醇建立小鼠脑损伤模型。方法 60只ICR小鼠分为6组，颅内注射不同剂量的无水乙醇，建立小鼠脑损伤模型。7 d后对各组小鼠脑组织做病理、丙二醛(MDA)、超氧化物歧化酶(SOD)和基因表达等检测。结果 15μL无水乙醇剂量组的死亡率为30%，造模成功率为100%;造模后各实验组初期表现为行走和平衡功能障碍，后期恢复正常，但采食量和体质量明显下降。脑损伤造模后，神经细胞出现坏死，周围出现水肿并伴有神经元细胞和神经胶质细胞的细胞间隙扩大;微血管外间隙扩大并且血管内有血细胞渗出;神经细胞和神经胶质细胞的出现空泡样病变。脑组织SOD活性下降和MDA含量显著升高。Q-PCR检测环氧合酶-2(Cox-2),血管内皮细胞生长因子(VEGF)和突触小体相关蛋白-25(SNAP-25) mRNA表达水平结果表明，与对照组相比，各实验组COX-2, VEGF和SNAP-25 mRNA的表达显著升高。结论 颅内注射无水乙醇建立小鼠脑损伤模型较传统大鼠脑损伤模型的建立操作简单、模型表型均一，重复性好，成功率高。更多还原

【Abstract】 Objective To establish a stable mouse model of brain injury by ethanol intracranial injection. Methods Sixty ICR mice were randomly divided into 6 groups: sham group(n=10), 5 μL group(n=10), 10 μL group(n=10), 15 μL group(n=10), 20 μL group(n=10) and 25 μL group(n=10).The mice were intranscranially injected with different doses of ethanol. After 7 days, pathological examination was performed and the level of malondialdehyde(MDA), superoxide dismutase(SOD)and relevant genes expression were detected. Results After model made, the ethanol injection mice showed walk and balance function obstacle at early time, and returned to normal later, but feed intake and body weight significantly decreased. With HE staining, the nerve cell necrosis and surrounding tissue edema, neurons and glial cells intercellular space expansion, microvascular clearance outside enlarge and vascular diapedesis, the nerve cells and glial cells cavity lesions were detected. The activity of SOD were decreased and content of MDA were increased. The cyclooxygenase-2(COX-2) and vascular endothelial growth factor(VEGF) and synaptosomal-associated protein-25(SNAP-25) mRNA expression was significantly increased in experimental group compared with control group, detected by Q-PCR.Conclusion The mouse model of brain injury made by intracranial injection with ethanol have the advantages of simple, phenotypic uniformity, good repeatability, high success rate compared to traditional brain injury model making.更多还原