【摘要】 目的 探讨慢性束缚应激(CRS)对小鼠认知功能的影响，观察海马不同亚区(CA1、CA3和DG区)星形胶质细胞(AS)的激活情况，明确慢性应激对海马的损伤是否存在特异性靶点。方法 雄性KM小鼠24只按体质量随机分为对照组和应激组，每组各12只。采用慢性束缚方法建立小鼠CRS模型，利用新异物体识别实验(NORT)和Morris水迷宫(MWM)评测其认知功能，HE染色观察细胞的形态结构变化，免疫组织化学染色法对海马CA1、CA3和DG区AS标志物胶质纤维酸性蛋白(GFAP)进行染色，采用显微镜和图像分析软件对海马上述亚区AS进行计数并分析GFAP的表达。结果 与对照组相比，应激组小鼠NORT及MWM实验的成绩均显著下降(P<0.05)，海马神经元形态结构损伤，CA1和CA3区AS细胞数及GFAP表达均显著增加(P<0.05),DG区两者均无显著变化(P>0.05)。结论 CRS能够导致小鼠认知功能障碍，海马CA1、CA3区AS的活化可能是其机制之一。更多还原

【Abstract】 Objective To explore the impact of chronic restraint stress(CRS) on the cognitive function of mice, and observe the activation of astrocyte(AS) in different subregions of hippocampus(CA1, CA3and DG), thus to make clear whether the damage of chronic stress in hippocampus has specific targets.Methods According to the body mass, 24 male Kunming mice were randomly divided into control group and stress group, with 12 mice in each group. A chronic stress model of mouse was established by using chronic restraint method. The cognitive function was evaluated by novel object recognition test(NORT)and Morris water maze(MWM), cell morphology changes were observed by HE staining, while the glial fibrillary acidic protein(GFAP) as marker of AS in hippocampal CA1, CA3 and DG areas were observed by immunohistochemistry staining. The AS number and GFAP expression in subregions of hippocampus were counted and analyzed by using the microscope and image analysis software respectively.Results Compared with the control group, the NORT and MWM scores of stress group were both decreased significantly(P<0.05), AS numbers and GFAP expression in CA1, CA3 areas were both significantly increased(P<0.05), and in DG area there was no significant changes(P>0.05).Conclusion CRS could lead to cognitive dysfunction in mice, and the activation of AS in hippocampus CA1, CA3 regions may be one of the mechanisms.更多还原